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e2f1 expression vector pcmv-e2f1  (Thermo Fisher)


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    Thermo Fisher e2f1 expression vector pcmv-e2f1
    Expression analysis for genes that as the G1-S phase and DNA replication regulators after RPL21 knockdown. (A,B) The validation of transcriptome analysis by quantitative real-time PCR (qPCR). Human pancreatic cancer PANC-1 cells were transfected with siL21-Mix (40 nM, 72 h), the up-regulated genes ( AHR , THBS1 , DDIT3 , and MKNK2 ) in transcriptome sequencing were confirmed by qPCR. The down-regulated genes ( <t>E2F1</t> , PCNA , CCND1 , CCNE1 MCM2 , MCM4 , MCM5 , MCM7 , and KIAA0101 ) in transcriptome sequencing were confirmed by qPCR. (C) The PANC-1 and BxPC-3 cells were transfected with Mock-siRNA, siL21-1 and siL21-2 (40 nM, 72 h). The equal amounts (15 μg) of each protein sample were analyzed by western blot with antibodies of E2F1 , CCND1 , CCNE1 , MCM2 -7 and GAPDH . The GAPDH served as an internal control. The control, negative control (NC), siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. Three independent experiments were of similar results.
    E2f1 Expression Vector Pcmv E2f1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e2f1 expression vector pcmv-e2f1/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    e2f1 expression vector pcmv-e2f1 - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "RPL21 siRNA Blocks Proliferation in Pancreatic Cancer Cells by Inhibiting DNA Replication and Inducing G1 Arrest and Apoptosis"

    Article Title: RPL21 siRNA Blocks Proliferation in Pancreatic Cancer Cells by Inhibiting DNA Replication and Inducing G1 Arrest and Apoptosis

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2020.01730

    Expression analysis for genes that as the G1-S phase and DNA replication regulators after RPL21 knockdown. (A,B) The validation of transcriptome analysis by quantitative real-time PCR (qPCR). Human pancreatic cancer PANC-1 cells were transfected with siL21-Mix (40 nM, 72 h), the up-regulated genes ( AHR , THBS1 , DDIT3 , and MKNK2 ) in transcriptome sequencing were confirmed by qPCR. The down-regulated genes ( E2F1 , PCNA , CCND1 , CCNE1 MCM2 , MCM4 , MCM5 , MCM7 , and KIAA0101 ) in transcriptome sequencing were confirmed by qPCR. (C) The PANC-1 and BxPC-3 cells were transfected with Mock-siRNA, siL21-1 and siL21-2 (40 nM, 72 h). The equal amounts (15 μg) of each protein sample were analyzed by western blot with antibodies of E2F1 , CCND1 , CCNE1 , MCM2 -7 and GAPDH . The GAPDH served as an internal control. The control, negative control (NC), siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. Three independent experiments were of similar results.
    Figure Legend Snippet: Expression analysis for genes that as the G1-S phase and DNA replication regulators after RPL21 knockdown. (A,B) The validation of transcriptome analysis by quantitative real-time PCR (qPCR). Human pancreatic cancer PANC-1 cells were transfected with siL21-Mix (40 nM, 72 h), the up-regulated genes ( AHR , THBS1 , DDIT3 , and MKNK2 ) in transcriptome sequencing were confirmed by qPCR. The down-regulated genes ( E2F1 , PCNA , CCND1 , CCNE1 MCM2 , MCM4 , MCM5 , MCM7 , and KIAA0101 ) in transcriptome sequencing were confirmed by qPCR. (C) The PANC-1 and BxPC-3 cells were transfected with Mock-siRNA, siL21-1 and siL21-2 (40 nM, 72 h). The equal amounts (15 μg) of each protein sample were analyzed by western blot with antibodies of E2F1 , CCND1 , CCNE1 , MCM2 -7 and GAPDH . The GAPDH served as an internal control. The control, negative control (NC), siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. Three independent experiments were of similar results.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Transfection, Sequencing, Western Blot, Negative Control

    RPL21 regulates the cell cycle and DNA replication via E2F1 in PANC-1 and BxPC-3 cells. (A,C) The luciferase reporter vectors ( CCND1 , CCNE1 and MCM2 - 7 ) were constructed using pGL-3 vectors. 1 μg reporter vector was co-transfected with 1 μg pCMV-E2F1 vector or 1 μg empty pcDNA3.1 vector (control) for each well (6-well plates) containing 6 × 10 5 PANC-1 and BxPC-3 cells. Luciferase activity was measured 24 h after the transfection. (B,D) The PANC-1 and BxPC-3 cells were transfected with siRNAs with lipofectamine 2000 reagent. The control, NC, siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. The cells after transfection were seeded in 6-well plates at 6 × 10 5 cells/well, and 2 g E2F1 luciferase reporters (E2F1-promoter) were transfected using lipofectamine 2000 reagent. Luciferase activity was measured 24 h after the transfection. Each bar represents the mean ± SD of triplicate analysis. * indicates P < 0.05 compared to the control as determined by the Student’s t -test.
    Figure Legend Snippet: RPL21 regulates the cell cycle and DNA replication via E2F1 in PANC-1 and BxPC-3 cells. (A,C) The luciferase reporter vectors ( CCND1 , CCNE1 and MCM2 - 7 ) were constructed using pGL-3 vectors. 1 μg reporter vector was co-transfected with 1 μg pCMV-E2F1 vector or 1 μg empty pcDNA3.1 vector (control) for each well (6-well plates) containing 6 × 10 5 PANC-1 and BxPC-3 cells. Luciferase activity was measured 24 h after the transfection. (B,D) The PANC-1 and BxPC-3 cells were transfected with siRNAs with lipofectamine 2000 reagent. The control, NC, siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. The cells after transfection were seeded in 6-well plates at 6 × 10 5 cells/well, and 2 g E2F1 luciferase reporters (E2F1-promoter) were transfected using lipofectamine 2000 reagent. Luciferase activity was measured 24 h after the transfection. Each bar represents the mean ± SD of triplicate analysis. * indicates P < 0.05 compared to the control as determined by the Student’s t -test.

    Techniques Used: Luciferase, Construct, Plasmid Preparation, Transfection, Activity Assay

    Model of E2F1 -mediated pancreatic cancer cell proliferation.
    Figure Legend Snippet: Model of E2F1 -mediated pancreatic cancer cell proliferation.

    Techniques Used:



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    e2f1 expression vector pcmv-e2f1  (Thermo Fisher)
    90
    Thermo Fisher e2f1 expression vector pcmv-e2f1
    Expression analysis for genes that as the G1-S phase and DNA replication regulators after RPL21 knockdown. (A,B) The validation of transcriptome analysis by quantitative real-time PCR (qPCR). Human pancreatic cancer PANC-1 cells were transfected with siL21-Mix (40 nM, 72 h), the up-regulated genes ( AHR , THBS1 , DDIT3 , and MKNK2 ) in transcriptome sequencing were confirmed by qPCR. The down-regulated genes ( <t>E2F1</t> , PCNA , CCND1 , CCNE1 MCM2 , MCM4 , MCM5 , MCM7 , and KIAA0101 ) in transcriptome sequencing were confirmed by qPCR. (C) The PANC-1 and BxPC-3 cells were transfected with Mock-siRNA, siL21-1 and siL21-2 (40 nM, 72 h). The equal amounts (15 μg) of each protein sample were analyzed by western blot with antibodies of E2F1 , CCND1 , CCNE1 , MCM2 -7 and GAPDH . The GAPDH served as an internal control. The control, negative control (NC), siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. Three independent experiments were of similar results.
    E2f1 Expression Vector Pcmv E2f1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e2f1 expression vector pcmv-e2f1/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    e2f1 expression vector pcmv-e2f1 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

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    Expression analysis for genes that as the G1-S phase and DNA replication regulators after RPL21 knockdown. (A,B) The validation of transcriptome analysis by quantitative real-time PCR (qPCR). Human pancreatic cancer PANC-1 cells were transfected with siL21-Mix (40 nM, 72 h), the up-regulated genes ( AHR , THBS1 , DDIT3 , and MKNK2 ) in transcriptome sequencing were confirmed by qPCR. The down-regulated genes ( E2F1 , PCNA , CCND1 , CCNE1 MCM2 , MCM4 , MCM5 , MCM7 , and KIAA0101 ) in transcriptome sequencing were confirmed by qPCR. (C) The PANC-1 and BxPC-3 cells were transfected with Mock-siRNA, siL21-1 and siL21-2 (40 nM, 72 h). The equal amounts (15 μg) of each protein sample were analyzed by western blot with antibodies of E2F1 , CCND1 , CCNE1 , MCM2 -7 and GAPDH . The GAPDH served as an internal control. The control, negative control (NC), siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. Three independent experiments were of similar results.

    Journal: Frontiers in Oncology

    Article Title: RPL21 siRNA Blocks Proliferation in Pancreatic Cancer Cells by Inhibiting DNA Replication and Inducing G1 Arrest and Apoptosis

    doi: 10.3389/fonc.2020.01730

    Figure Lengend Snippet: Expression analysis for genes that as the G1-S phase and DNA replication regulators after RPL21 knockdown. (A,B) The validation of transcriptome analysis by quantitative real-time PCR (qPCR). Human pancreatic cancer PANC-1 cells were transfected with siL21-Mix (40 nM, 72 h), the up-regulated genes ( AHR , THBS1 , DDIT3 , and MKNK2 ) in transcriptome sequencing were confirmed by qPCR. The down-regulated genes ( E2F1 , PCNA , CCND1 , CCNE1 MCM2 , MCM4 , MCM5 , MCM7 , and KIAA0101 ) in transcriptome sequencing were confirmed by qPCR. (C) The PANC-1 and BxPC-3 cells were transfected with Mock-siRNA, siL21-1 and siL21-2 (40 nM, 72 h). The equal amounts (15 μg) of each protein sample were analyzed by western blot with antibodies of E2F1 , CCND1 , CCNE1 , MCM2 -7 and GAPDH . The GAPDH served as an internal control. The control, negative control (NC), siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. Three independent experiments were of similar results.

    Article Snippet: The E2F1 expression vector pCMV-E2F1 was constructed using the pcDNA3.1 plasmid (Invitrogen, Carlsbad, CA, United States).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Transfection, Sequencing, Western Blot, Negative Control

    RPL21 regulates the cell cycle and DNA replication via E2F1 in PANC-1 and BxPC-3 cells. (A,C) The luciferase reporter vectors ( CCND1 , CCNE1 and MCM2 - 7 ) were constructed using pGL-3 vectors. 1 μg reporter vector was co-transfected with 1 μg pCMV-E2F1 vector or 1 μg empty pcDNA3.1 vector (control) for each well (6-well plates) containing 6 × 10 5 PANC-1 and BxPC-3 cells. Luciferase activity was measured 24 h after the transfection. (B,D) The PANC-1 and BxPC-3 cells were transfected with siRNAs with lipofectamine 2000 reagent. The control, NC, siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. The cells after transfection were seeded in 6-well plates at 6 × 10 5 cells/well, and 2 g E2F1 luciferase reporters (E2F1-promoter) were transfected using lipofectamine 2000 reagent. Luciferase activity was measured 24 h after the transfection. Each bar represents the mean ± SD of triplicate analysis. * indicates P < 0.05 compared to the control as determined by the Student’s t -test.

    Journal: Frontiers in Oncology

    Article Title: RPL21 siRNA Blocks Proliferation in Pancreatic Cancer Cells by Inhibiting DNA Replication and Inducing G1 Arrest and Apoptosis

    doi: 10.3389/fonc.2020.01730

    Figure Lengend Snippet: RPL21 regulates the cell cycle and DNA replication via E2F1 in PANC-1 and BxPC-3 cells. (A,C) The luciferase reporter vectors ( CCND1 , CCNE1 and MCM2 - 7 ) were constructed using pGL-3 vectors. 1 μg reporter vector was co-transfected with 1 μg pCMV-E2F1 vector or 1 μg empty pcDNA3.1 vector (control) for each well (6-well plates) containing 6 × 10 5 PANC-1 and BxPC-3 cells. Luciferase activity was measured 24 h after the transfection. (B,D) The PANC-1 and BxPC-3 cells were transfected with siRNAs with lipofectamine 2000 reagent. The control, NC, siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. The cells after transfection were seeded in 6-well plates at 6 × 10 5 cells/well, and 2 g E2F1 luciferase reporters (E2F1-promoter) were transfected using lipofectamine 2000 reagent. Luciferase activity was measured 24 h after the transfection. Each bar represents the mean ± SD of triplicate analysis. * indicates P < 0.05 compared to the control as determined by the Student’s t -test.

    Article Snippet: The E2F1 expression vector pCMV-E2F1 was constructed using the pcDNA3.1 plasmid (Invitrogen, Carlsbad, CA, United States).

    Techniques: Luciferase, Construct, Plasmid Preparation, Transfection, Activity Assay

    Model of E2F1 -mediated pancreatic cancer cell proliferation.

    Journal: Frontiers in Oncology

    Article Title: RPL21 siRNA Blocks Proliferation in Pancreatic Cancer Cells by Inhibiting DNA Replication and Inducing G1 Arrest and Apoptosis

    doi: 10.3389/fonc.2020.01730

    Figure Lengend Snippet: Model of E2F1 -mediated pancreatic cancer cell proliferation.

    Article Snippet: The E2F1 expression vector pCMV-E2F1 was constructed using the pcDNA3.1 plasmid (Invitrogen, Carlsbad, CA, United States).

    Techniques: